Lastly, one??Ci of 86Rb was added to every culture and incubation was ongoing at 37?°C for yet another 10?min. Reactions ended up terminated by washing the cells with ice-cold 100?mM MgCl2 and mobile lysates were geared up for liquid scintillation counting. The 86Rb counts in parallel cultures with ouabain were subtracted from People without havin